EC Number   |
|---|
   1.21.3.3 | - |
| 1.21.3.3 | canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid |
| 1.21.3.3 | expressed in Escherichia coli BL21 |
| 1.21.3.3 | expressed in Komagataella pastoris |
| 1.21.3.3 | expressed in Pichia pastoris strain GS115 |
| 1.21.3.3 | expressed in Spodoptera frugiperda (Sf9) |
| 1.21.3.3 | expressed in Spodoptera frugiperda Sf9 cells |
| 1.21.3.3 | expression in Pichia pastoris |
| 1.21.3.3 | expression in Pichia pastoris strain KM71H |
| 1.21.3.3 | for co-overexpression with disulfide isomerase in Pichia pastoris cells, for cloning of wild-type BBE, the mutant H104A and mutant H104A-C166A the vector pPICZalpha is used |
