EC Number   |
|---|
   1.14.19.5 | - |
| 1.14.19.5 | C-terminal His-tagged fusion protein expressed using recombinant baculoviruses |
| 1.14.19.5 | DNA and amino acid sequence determination and analysis, recombinant expression of the enzyme as fusion protein with beta-actin from Mamestra brassicae, UniProt ID B0LF73, quantitative real-time PCR enzyme expression analysis |
| 1.14.19.5 | expressed as polyhistidine-tagged protein in elongase 1 and DELTA9 desaturase-deficient yeast cells |
| 1.14.19.5 | expressed in Sf9 insect cells |
| 1.14.19.5 | expressed in yeast |
| 1.14.19.5 | expression in Saccharomyces cerevisiae DELTAelo1/DELTAole mutant strain, which is both elongase 1 and DELTA9 desaturase-deficient, using a Cu+2 inducible expression vector, the recombinant expression leads to producing large quantities of C11-monounsaturated fatty acids, mainly (Z)-11-hexadecenoic acid, (E,E)-10,12-tetradecadienoic acid, minor amounts of (E,Z)-10,12-hexadecadienoic acid, and very low amounts of (E,Z)-10,12-tetradecadienoic isomer |
| 1.14.19.5 | expression in yeast |
| 1.14.19.5 | gene d2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of MsexAPTQ desaturase in Saccharomyces cerevisiae strain W303 |
| 1.14.19.5 | genetic trans-formation of a desaturase-deficient strain of the yeast Sac-charomyces cerevisiae with an expression plasmid encoding PDesat-Tn DELTA11Z results in complementation of the strain's fatty acid auxotrophy and the production of DELTA11 Z-unsaturated fatty acids |
