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Results 1 - 10 of 10
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8a steroid 15beta-hydroxylating whole-cell solvent tolerant biocatalyst is constructed by expressing the Bacillus megaterium steroid hydroxylase CYP106A2 in the solvent tolerant Pseudomonas putida S12 699068
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8CYP106A2 can be easily expressed in Escherichia coli with a high yield and can be reconstituted using the adrenal redox proteins, adrenodoxin and adrenodoxin reductase 693956
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8expressed in Escherichia coli and Bacillus subtilis. No hydroxylation is found with protein extracts from recombinant Escherichia coli strains since cytochrome P450meg needs additional electron transfer proteins for enzymatic activity, which are missing in Escherichia coli. Bacillus subtilis, in contrast to Escherichia coli, contains an electron transfer system capable of supporting the activity of cytochrome P450meg 700219
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8expression in Escherichia coli 695981, 697323, 744148, 745540
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8expression in Escherichia coli BL21DE3 695980
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8expression in Escherichia coli, wild type and mutant S72A/V73I, the DELTA72 mutant, which lacks the first 72 amino acids, is not expressed in Escherichia coli at a detectable amount, suggesting that the truncated mutant cannot fold properly within the bacterial cell 696142
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8expression of the enzyme together with the electron-transfer partners bovine adrenodoxin and adrenodoxin reductase in Escherichia coli. Additionally an enzyme-coupled cofactor regeneration system was implemented by expressing alcohol dehydrogenase from Lactobacillus brevis. By studying the conversion of progesterone and testosterone, the bottlenecks of these P450-catalyzed hydroxylations are identified. Substrate transport into the cell and substrate solubility turned out to be crucial for the overall performance. Based on these investigations a new concept for CYP106A2-catalyzed steroid hydroxylations is developed by which the productivity of progesterone and testosterone conversion could be increased up to 18fold to yield an absolute productivity up to 5.5 g/L*d 697322
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8exression in Bacillus megaterium 745699
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8functional expression of CYP106A2 in Escherichia coli strain BL21 from plasmid pET-CYP13 on the outer membrane with exposure on the surface without the external addition of the heme group but absolutely requiring the coexpression of TolC channel protein JW5503, because Escherichia coli uses a TolC-dependent mechanism to export heme into the growth media, where it can be scavenged by a surface-displayed apoenzyme 725584
Display the word mapDisplay the reaction diagram Show all sequences 1.14.15.8recombinant expression of CYP106A2 by protoplast transformation is only successfully in the plasmid-less Bacillus megaterium strain MS941, not in strain ATCC 13368, coexpression of heterologous redox chain of the P450, bovine adrenodoxin reductase, and bovine adrenodoxin 724061
Results 1 - 10 of 10