EC Number   |
|---|
   1.14.15.16 | expressed in chinese hamster lung fibroblast cells (V79-4 cells) |
| 1.14.15.16 | expressed in Escherichia coli |
| 1.14.15.16 | expressed in Escherichia coli BL21-Gold(DE3) and Rosetta 2(DE3) cells |
| 1.14.15.16 | expressed in Escherichia coli JM109 cells |
| 1.14.15.16 | expressed in Sf21 insect cells using the previously cloned cDNA in baculovirus (AcNPV-P450cc24), solubilized mitochondrial protein from Sf21 cells infected with recombinant virus AcNPV-P450cc24 cDNA express 24-hydroxylase activity when reconstituted with bovine adrenodoxin, adrenodoxin reductase, and NADPH |
| 1.14.15.16 | expressed in V79-CYP24 or OK (CCL-1840) cells |
| 1.14.15.16 | gene CYP24A1, cloned from leukocyte DNA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR, recombinant expression of wild-type and mutant CYP24A1 cDNAs in JEG-3 cells. The mammalian two-hybrid assay system consisting of the retinoid-X receptor and vitamin D receptor in JEG-3 cells is used as a simple, sensitive, quantitative assay of enzyme activity |
| 1.14.15.16 | gene CYP24A1, functional recombinant His-tagged enzyme expression in Escherichia coli membranes |
| 1.14.15.16 | gene CYP24A1, overexpression of N-terminally maltose-binding-protein fused enzyme in Escherichia coli |
| 1.14.15.16 | gene CYP24A1, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH5alpha F'IQ |
