EC Number   |
|---|
   1.14.14.21 | gene bdsC, DNA and amino acid sequence determination and analysis, recombinant functional expression in Escherichia coli strain JM109, coexpression with a flavin reductase Frb, encoded by gene frb, UniProt ID Q75V96. Coexpression of frb with the DBT-desulfurization genes (bdsABC) from Bacillus subtilis strain WU-S2B is critical for high DBT-desulfurizing ability over a wide temperature range of 20-55°C, coexpression screening using indigo production as selective indication |
| 1.14.14.21 | gene bdsC, recombinant expression in Escherichia coli strain BL21(DE3)/pET21-a/bdsC |
| 1.14.14.21 | gene bdsC, recombinant overexpression in Escherichia coli |
| 1.14.14.21 | gene dszC |
| 1.14.14.21 | gene dszC, cloning from dszABC operon, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis |
| 1.14.14.21 | gene dszC, cloning from strain DS-3, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) |
| 1.14.14.21 | gene dszC, DNA and amino acid sequence determination and analysis, cloning and recombinant expression in Escherichia coli strain JM109 |
| 1.14.14.21 | gene dszC, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha |
| 1.14.14.21 | gene dszC, functional recombinant enzyme expression in Thermus thermophilus strain HB27 |
| 1.14.14.21 | gene dszC, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
