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EC Number	Cloned (Commentary)
1.14.14.176	cDNAs encoding taxa-4(5),11(12)-diene synthase and CYP725A4 are introduced in Nicotiana sylvestris for specific expression in trichome cells. Production of truncated TS in Escherichia coli. The TS cDNA coding for a protein truncated at the N-terminus at amino acid position 75 is cloned in the pET-30b expression vector. Furthermore, CYP725A4 is expressed in yeast.
1.14.14.176	CYP725A4, DNA and amino acid sequence determination and analysis, expression in the Saccharomyces cerevisiae WAT11 strain containing the Arabidopsis reductase ATR1, and co-expression in Nicotiana sylvestris with taxa-4(5),11(12)-diene synthase, which does not lead to 5alpha-hydroxytaxa-4(20),11(12)-diene, but to formation of 5(12)-oxa-3(11)-cyclotaxane, a tritiated oxidized taxane, structural analysis of the compound, phenotype, overview
1.14.14.176	expressed in Escherichia coli BL21 (DE3) cells
1.14.14.176	expressed in Escherichia coli BL21(DE3) cells
1.14.14.176	expressed in Escherichia coli C41(DE3) cells
1.14.14.176	expressed in Saccharomyces cerevisiae and Escherichia coli

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Release 2023.1 (January 2023)