EC Number |
---|
1.14.13.92 | - |
1.14.13.92 | BVMO gene, functional expression in Escherichia coli strains JM109 and BL21(DE3) as soluble protein |
1.14.13.92 | expressed in Escherichia coli BL21(DE3) cells |
1.14.13.92 | expressed in Escherichia coli Top10 cells |
1.14.13.92 | expression in Escherichia coli |
1.14.13.92 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli |
1.14.13.92 | expression of His-tagged wild-type and mutant PAMOs in Escherichia coli strain TOP10 |
1.14.13.92 | gene pamO, recombinant expression of His-tagged enzyme in three different forms (C, N, and L) concerning the position of His-tag in Escherichia coli strain BL21(DE3), the His-tag locates at the N- and C-termini of the enzyme (PAMO N and PAMO C, respectively), PAMO L describes a construct with the His-tag distanced from the enzyme C-terminus with an additional 19-amino acid sequence, the enzyme forms do not show great differences in catalytic activity. No use of the arabinose promoter in the expression system |
1.14.13.92 | overexpressed in Escherichia coli |
1.14.13.92 | overexpressed via plasmid in Escherichia coli |