EC Number   |
|---|
   1.14.13.111 | cloning of the reductase encoded by the msmD gene. Cloning and overexpression of the msmD gene, encoding the reductase component, as a GST fusion protein results in the expression of a 65000 Da polypeptide matching the size of the GST protein plus the reductase protein when induced with isopropyl thio-beta-D-galactoside. The fusion between the two proteins is unstable, and purification by affinity chromatography is not possible |
| 1.14.13.111 | expression in Escherichia coli. The four polypeptides comprising MSAMO are the products of the coordinated expression of an operon (msmABCD) |
| 1.14.13.111 | gene encoding the electron transfer protein (MsmC) of methanesulfonic acid monooxygenase |
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha |
| 1.14.13.111 | msm gene cluster. Development of functional gene probes centered around the unique Rieske center encoding region of msmA which can be used to detect the presence of methanesulfonate-utilizing bacteria in the environment |
