EC Number   |
|---|
   1.14.12.3 | expressed in Escherichia coli JM109 |
| 1.14.12.3 | expression in Escherichia coli JM109 |
| 1.14.12.3 | expression in Escherichia coli strain DH5alpha and JM109, co-expression with benzene dihydrodiol dehydrogenase under the control of the Ptac promoter or without any induction. The recombinant strains expressing the BED and the BDDH enzymes transform benzene into dihydrodiol with corresponding consumption of oxygen and regenerate NADH by converting dihydrodiol to catechol |
| 1.14.12.3 | expression of the alpha-subunit and the beta-subunit of terminal dioxygenase in Escherichia coli |
| 1.14.12.3 | expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and CJ236 |
| 1.14.12.3 | genes bedC1 and bedC2 encoding the terminal oxygenase alpha-subunit and beta-subunit, expression in Escherichia coli |
| 1.14.12.3 | genes bedC1C2BA, under the control of the tac promoter, subcloned into pLAFR5, successfully conjugated into seven of the Gram-negative cis-1,2-cyclohexanediol-degrading isolates and stably maintained and expressed in three of them, strain CHD1ΒCHD3, the strains grow on cis-1,2-cyclohexanediol as sole carbon source, express an active BDO and oxidise cyclohexene, but none of the three strains is able to grow on cyclohexene as sole carbon source, overview, expression in Escherichia coli strain JM109 |
