EC Number   |
|---|
    1.1.1.298 | expression in Escherichia coli Rosetta 2(DE3) |
| 1.1.1.298 | expression in Escherichia coli strain BL21 |
| 1.1.1.298 | gene mcr, functional expression in Escherichia coli under the T5 promoter control leading to production of 3-hydroxypropionate, coexpression with the nicotinamide nucleotide transhydrogenase that converts NADH to NADPH, required by MCR |
| 1.1.1.298 | gene mcr, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of acetyl-CoA synthetase |
| 1.1.1.298 | gene mcr, recombinant expression of MCR enzyme mutant in Escherichia coli strain CE43(DE3), coexpression with malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris, native transhydrogenase (PntAB), and NAD kinase (YfjB) |
| 1.1.1.298 | gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha |
| 1.1.1.298 | gene mcr, separate recombinant expression of the N- and C-terminal subdomains of the enzyme, MCR-C and MCR-N, in Schizosaccharomyces pombe strain FY12804/NBRP, coexpression of acetyl-CoA synthase, gene acsSE, and aldehyde dehydrogenase, gene atd1, under the control of the cam1 promoter |
| 1.1.1.298 | MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha |
