EC Number |
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1.1.1.270 | 3-ketosteroid reductase activity of the human 3(alpha-beta)-hydroxysteroid epimerase, stable expression |
1.1.1.270 | DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) pLysS |
1.1.1.270 | expression of HSD17B7 and truncated HSD17B7 (shortened by 127 amino acids at the C terminus) as glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium |
1.1.1.270 | expression of HSD17B7 as a glutathione-S-transferase fusion protein in Escherichia coli. Erg27p-deficient yeast strain complements the 3-ketosteroid reductase deficiency of the cells and restores growth on sterol-deficient medium |
1.1.1.270 | expression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system |
1.1.1.270 | heterologous expression in Escherichia coli |
1.1.1.270 | isoform type III of 3beta-hydroxysteroid dehydrogenase/DELTA5-DELTA4 isomerase with almost exclusive 3-ketosteroid reductase activity, cDNA expression |
1.1.1.270 | overexpression in Escherichia coli |
1.1.1.270 | overexpression in Escherichia coli BL21 (DE3) Codon Plus RP cells |
1.1.1.270 | quantitative expression analysis of AKR1C isozymes |