EC Number |
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1.1.1.202 | a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli |
1.1.1.202 | all genes of the dha regulon, including the gene for 1,3-propanediol oxidoreductase of Klebsiella pneumoniae mobilized by the plasmid RP4:mini Mu and transferred to Escherichia coli |
1.1.1.202 | expressed in Escherichia coli BL21 cells |
1.1.1.202 | expression in Clostridium acetobutylicum mutant strain DG1 from plasmid pSPD5, subcloning in Escherichia coli |
1.1.1.202 | expression in Escherichia coli |
1.1.1.202 | gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) |
1.1.1.202 | gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons |
1.1.1.202 | gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain JM109 |
1.1.1.202 | gene dhaT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in YqhD-deficient Escherichia coli strain BL21(DE3) |
1.1.1.202 | gene dhaT, expression of the N-terminally His-tagged in Escherichia coli strain BL21(DE3) |