EC Number   |
|---|
   1.1.1.12 | derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells |
| 1.1.1.12 | expression in Escherichia coli |
| 1.1.1.12 | gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
| 1.1.1.12 | gene lad1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.1.1.12 | gene ladA |
| 1.1.1.12 | gene ladA, DNA and amino acid sequence determination and analysis |
| 1.1.1.12 | gene ladA, DNA and amino acid sequence determination and analysis, construction of strain 3M-43/pyrA, a pyrA mutant of strain 3M-43, in which an intact copy of ladA is inserted under control of the Aspergillus nidulans gpdA promoter. Complementation of the 3M-43 pyrA strain with pGpd:ladA |
| 1.1.1.12 | gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.1.1.12 | gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.1.1.12 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
