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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 4.2.3.229expressed in Nicotiana benthamiana and Escherichia coli
Display the reaction diagram Show all sequences 4.2.3.229gene OsKSL2, full-length enzyme DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21
Display the reaction diagram Show all sequences 4.2.3.229isozyme determination and phylogenetic analysis and tree, sequence comparisons, overview. Synthetic RcKS(L) isozymes are truncated to remove the N-terminal plastid-directing transit peptide sequences, they are individually subcloned into compatible expression vectors and each coexpressed with either the geranylgeranyl phosphate synthase (GGPS), or the GGPS along with a CPS (EC 5.5.1.13) for recombinant expression in Escherichia coli
Results 1 - 3 of 3