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Results 1 - 9 of 9
EC Number
Commentary
Reference
expressed in Escherichia coli BL21 (DE3) and JM109 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
expressed in Escherichia coli Rosetta-gami 2 cells
gene HS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli; gene HS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli; gene HS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli
gene ZSS1 or hum, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) under control of the lac promoter
gene ZSS1 or HUM, recombinant His-tagged enzyme expression from codon-optimized alpha-humulene synthase gene in Escherichia coli strain BL21(DE3)
gene zssI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet from codon-optimized gene zssI with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1, cloning scheme comprising genes zssI and ERG20
gene ZSSI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet with alpha-humulene 8-hydroxylase (CYP71BA1) and zerumbone synthase variant (ZSD1S114A) from Zingiber zerumbet, together with AtCPR1 from Arabidopsis thaliana in Saccharomyces cerevisiae strain LW16, subcloning and expression in Escherichia coli strain trans-T1
Results 1 - 9 of 9