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EC Number
expression as a GST-fusion protein in Saccharomyces cerevisiae
expression in Escherichia coli
into the Gateway entry vector pENTR3C, for expressing Ape2 proteins in the yeast strain BJ5464, the cDNAs are cloned in fusion with GST using the plasmid pBJ842
recombinant expression of wild-type and mutant enzymes
the enzyme containing the mitochondrial targeting signal of manganese superoxide dismutase is expressed in a human malignant breast epithelial cell line, MDA-MB-231, this expression results in a decrease in cell viability following exposure to oxidative stress
Results 1 - 6 of 6