EC Number |
Reference |
---|
2.3.1.75 | cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants |
487487 |
2.3.1.75 | expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |
657612 |
2.3.1.75 | chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells |
659670 |
2.3.1.75 | MFAT, DNA and amino acid sequence determination and analysis, localized on the X chromosome, expression of the N-terminally FLAG-tagged enzyme in Spodoptera frugierda Sf9 cells using the baculovirus transfection system, or in COS-7 cells |
659670 |
2.3.1.75 | functional expression in Escherichia coli strain BL21(DE3) |
671446 |
2.3.1.75 | gene atfA, DNA and amino acid sequence determination and analysis, WS/DGAT family sequence comparisons and phylogenetic relationships, overview, expression in Escherichia coli as untagged or C-terminally His6-tagged enzyme |
672451 |
2.3.1.75 | genes with high homologies to the Acinetobacter sp. ADP1 atfA are identified in the genome databases of several Gram-negative strains, including marine bacteria like Hahella chejuensis, psychrophilic strains like Psychrobacter sp., Polaromonas sp. and Bradyrhizobium japonicum. Ten atfA homologous genes are identified in the genome database of Arabidopsis thaliana |
672451 |
2.3.1.75 | screening, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 |
674328 |
2.3.1.75 | genes atfA1 and atfA2, expression in Escherichia coli strain BL21(DE3) |
674345 |
2.3.1.75 | gene AWAT1, i.e. DGA2, location on the X chromosome Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae |
674424 |