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EC Number
Cloned (Commentary)
Reference
expressed as C-terminally His6-tagged enzyme, wild-type and mutant enzymes C125A, C129A and C132A
expressed in Escherichia coli Rosetta2(DE3)pLysS cells
gene cfr, operon isc, co-expression with isc proteins, expression of His6-tagged Cfr under control of an arabinose-inducible promoter in Escherichia coli strain BL21Star
gene cfr, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain +Rosetta2(DE3)
gene cfr, sequence comparisons and phylogenetic analysis, primer extension analysis, cloning and expression in Escherichia coli strains TOP10 and AS19
gene cfr, The Cfr-like protein in Peptoclostridium difficile is hosted by a transposon, and the same gene is found in other strains, sequence comparisons, overview. Recombinant expression in Escherichia coli strain AS19 which then shows elevated MIC values for five classes of antibiotics, and recombinant expression in rlmN-deficient Escherichia coli strain JW2505-1
gene cfr, while cfr is typically plasmid borne, in CM05 it is located on the chromosome and is coexpressed with ermB as part of the mlr operon. DNA and amino acid sequence determination and analysis and genetic organization, overview. The cfr-containing mlr operon is located within a 15.5-kb plasmid-like insertion into 23S rRNA allele 4. In the LZDs colonies, designated CM05DELTA, a recombination event involving the two ermB genes had occurred, resulting in the deletion of cfr and the 3= flanking region, cfr-istAS-istBS-ermB
gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
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