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Cloned (Commentary)
expression of C-terminally His6-tagged full-length enzyme, expression of the C-terminal domain and the N-terminal domain as SUMO fusion proteins, the C-terminal domain is unstable after detagging through ubiquitin-like-specific protease 1 cleavage, while the N-terminal domain is stable
recombinant YgjO protein carrying a C-terminal GFP-His6-tag is expressed in Escherichia coli
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