EC Number   |
Subunits |
Reference |
|---|
   2.3.1.168 | 24-mer |
24 * 52000, SDS-PAGE |
644842 |
| 2.3.1.168 | ? |
x * 52600, SDS-PAGE |
644839, 644840, 644841 |
| 2.3.1.168 | ? |
x * 54000, SDS-PAGE |
644848 |
| 2.3.1.168 | More |
assembly of 24 E2 subunits into a cubic structure, forming the core of the mammalian branched-chain alpha-keto acid dehydrogenase multienzyme complex |
644842 |
| 2.3.1.168 | More |
C-terminus of E2 is joined to the N-terminus of E3 in the pyruvate dehydrogenase complex, subunit organization |
644846 |
| 2.3.1.168 | More |
enzyme shows the E2 structure of 3 folded domains: lipoyl-bearing, E3-binding, and inner core, typical for all E2 protein of alpha-keto acid dehydrogenases |
644842 |
| 2.3.1.168 | More |
in vitro reconstitution of the 24-meric E2 inner core requires the chaperonins GroEL and GroES |
644849 |
| 2.3.1.168 | More |
lipoate-free inner E2 core: 26 kDa fragment contains the active site, 22 kDa fragment B is the subunit-binding domain, fragments are gained by tryptic digest |
644841 |
| 2.3.1.168 | More |
lipoyl domains and inner core in the structure of multienzyme complex, overview |
644846 |
| 2.3.1.168 | More |
recombinant apo-E2 is unable to reconstitute with recombinant E1 and E3 to an active branched-chain alpha-keto dehydrogenase, but recombinant holo-E2 is able to |
644847 |
