EC Number   |
Subunits |
Reference |
|---|
    1.2.1.32 | ? |
x * 51504, nucleotide sequence |
390256 |
| 1.2.1.32 | ? |
x * 51682, nucleotide sequence |
-, 247050 |
| 1.2.1.32 | ? |
x * 51700, nucleotide sequence |
247048 |
| 1.2.1.32 | ? |
x * 56252, recombinant N-terminally His-tagged enzyme mutant E268A, mass spectrometry |
-, 743338 |
| 1.2.1.32 | dimer |
2 * 57000, SDS-PAGE |
-, 389581 |
| 1.2.1.32 | More |
analysis of crystal structures of a tetrahedral, thiohemiacetal intermediate, a thioacyl intermediate and an NAD+-bound complex from the active site mutant E268A. These covalent intermediates are characterized by single-crystal and solution-state electronic absorption spectroscopy. The crystal structures reveal that the substrate undergoes an E/Z isomerization at the enzyme active site before an sp3-to-sp2 transition during enzyme-mediated oxidation |
-, 743338 |
| 1.2.1.32 | trimer |
3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence |
-, 390254 |
