EC Number   |
Subunits  |
Reference |
|---|
    1.14.15.3 | ? |
x * 40100, amino acid composition |
285365 |
| 1.14.15.3 | ? |
x * 42000, SDS-PAGE |
285363, 285364, 744575 |
| 1.14.15.3 | ? |
x * 42100, calculated for isoform AlkB1, x * 41600, calculated for isoform AlkB2 |
-, 746249 |
| 1.14.15.3 | ? |
x * 44000-46000, recombinant StrepII-tagged AlkB, SDS-PAGE |
-, 723970 |
| 1.14.15.3 | ? |
x * 46000, recombinant Strep-tagged AlkB, SDS-PAGE |
-, 724025 |
| 1.14.15.3 | ? |
x * 48000. SDS-PAGE |
745115 |
| 1.14.15.3 | More |
the alkane hydroxylase system consists of the alkane hydroxylase large subunit, the alkane hydroxylase small subunit, the NADH-dependent reductase subunit, and the ferredoxin subunit |
725284 |
| 1.14.15.3 | More |
the physiologically active form of membrane-embedded AlkB may be a multimer, the monomeric form of AlkB produced by purification in n-decyl-beta-D-maltopyranoside, n-dodecyl-beta-D-maltopyranoside, octyl glucose neopentyl glycol, and n-dodecyl-N,N-dimethylamine-N-oxide is largely inactive. In contrast, detergents that do not disrupt the AlkB oligomeric state, i.e. decyl maltose neopentyl glycol, lauryl maltose neopentyl glycol, and octaethylene glycol monododecyl ether, preserve the enzyme activity at a level close to that of the detergent-free control sample |
-, 724025 |
| 1.14.15.3 | trimer |
3 * 53000, recombinant His6-tagged alkane hydroxylase large subunit, SDS-PAGE |
725284 |
| 1.14.15.3 | trimer |
AlkB reconstituted into a lipid bilayer forms trimers |
-, 724025 |
