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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii (ribonucleotide)20 + dGMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii DSM 12428 (ribonucleotide)20 + dGMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + GTP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii (ribonucleotide)20 + GMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + GTP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii DSM 12428 (ribonucleotide)20 + GMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + ITP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii (ribonucleotide)20 + IMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + ITP in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP Pyrococcus horikoshii DSM 12428 (ribonucleotide)20 + IMP + diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP - Thermus thermophilus (ribonucleotide)40 + GMP + diphosphate overall reaction ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP - Thermobifida fusca (ribonucleotide)40 + GMP + diphosphate overall reaction, phosphodiester bond synthesis is rate limiting ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrococcus horikoshii (ribonucleotide)n+m - ?
Display the word mapDisplay the reaction diagram Show all sequences 6.5.1.8(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease Pyrobaculum aerophilum (ribonucleotide)n+m RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required ?
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