EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   6.3.2.32 | ATP + coenzyme gamma-F420-2 + L-glutamate |
the enzyme caps the gamma-glutamyl tail of the hydride carrier coenzyme F420. CofF specifically adds an alpha-linked glutamate to gamma-F420-2 produced by the Methanococcus jannaschii CofE protein. Coenzyme F420-2 is coenzyme F420 with two glutamic acid residue, coenzyme F420-3 is coenzyme F420 with three glutamic acid residues |
Methanocaldococcus jannaschii |
ADP + phosphate + coenzyme alpha-F420-3 |
- |
? |
| 6.3.2.32 | ATP + coenzyme gamma-F420-2 + L-glutamate |
CofF specifically adds an alpha-linked glutamate to gamma-F420-2 produced by the Methanococcus jannaschii CofE protein. Coenzyme F420-2 is coenzyme F420 with two glutamic acid residue, coenzyme F420-3 is coenzyme F420 with three glutamic acid residues |
Methanocaldococcus jannaschii |
ADP + phosphate + coenzyme alpha-F420-3 |
- |
? |
| 6.3.2.32 | GTP + coenzyme gamma-F420-2 + L-glutamate |
activity with GTP is 25% of the activity with ATP at 5 mM. CofF specifically adds an alpha-linked glutamate to gamma-F420-2 produced by the Methanococcus jannaschii CofE protein. Coenzyme F420-2 is coenzyme F420 with two glutamic acid residue, coenzyme F420-3 is coenzyme F420 with three glutamic acid residues |
Methanocaldococcus jannaschii |
GDP + phosphate + coenzyme alpha-F420-3 |
- |
? |
| 6.3.2.32 | more |
the phosphonate nucleotide analogs alpha,beta-CH2-ATP and beta,gamma-CH2-ATP support no ligase activity at concentrations of 5 mM. None of the following amino acids or analogs support ligase activity at 10-mM concentrations: D-glutamate, beta-glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or D,L-2-amino-4-phosphono-butyrate |
Methanocaldococcus jannaschii |
? |
- |
? |
