EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
- |
Comamonas testosteroni |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
- |
? |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
- |
Brevundimonas vesicularis |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
- |
? |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
extracts of Escherichia coli JM109 carrying pRE718 catalyze the conversion of trans-o-hydroxybenzylidenepyruvate or 2-hydroxychromene-2-carboxylate to an equilibrium mixture that contained 55% 2-hydroxychromene-2-carboxylate and 45% trans-o-hydroxybenzylidenepyruvate at pH 7. At pH 10 the reaction occus entirely in on direction, the conversion of 2-hydroxychromene-2-carboxylate to trans-o-hydroxybenzylidenepyruvate. The product is identified by nuclear magnetic resonance spectroscopy. This isomerization occurs spontaneously, although at a slower rate than the enzyme-catalyzed reaction |
Pseudomonas putida |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate |
r |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
glutathione (GSH)-dependent interconversion. The isomerization reaction involves a short-lived covalent adduct between the sulfur of GSH and C7 of the substrate |
Pseudomonas putida |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate |
? |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum |
Pseudomonas sp. |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate |
? |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate |
the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum |
Pseudomonas sp. TA-2 |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate |
i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate |
? |
5.99.1.4 | 2-hydroxybenzo[g]chromene-2-carboxylate |
more unstable than 2-hydroxychromene-2-carboxylate |
Comamonas testosteroni |
? |
- |
? |
5.99.1.4 | 2-hydroxybenzo[g]chromene-2-carboxylate |
more unstable than 2-hydroxychromene-2-carboxylate |
Brevundimonas vesicularis |
? |
- |
? |
5.99.1.4 | more |
enzyme degrades naphthalenesulfonates |
Comamonas testosteroni |
? |
- |
? |
5.99.1.4 | more |
enzyme degrades naphthalenesulfonates |
Brevundimonas vesicularis |
? |
- |
? |