EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate |
- |
Gaeumannomyces graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-10,12-octadecadienoate |
- |
Gaeumannomyces graminis var.graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxyoctadeca-9,12-dienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
- |
Gaeumannomyces graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group |
Gaeumannomyces graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
bifunctional enzyme with linoleic acid 8R-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group |
Gaeumannomyces graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
expression in Pichia pastoris changes the position and stereospecificity of a hydroperoxide isomerase (the recombinant enzyme forms 5,8(R)-dihydroxylinoleic acid (60% 5S) and 8(R),13-dihydroxyoctadeca-(9E,11E)-dienoic acid) possibly due to N- or O-linked mannosides in the vicinity of the heme group, whereas the (8R)-dioxygenase activity is identical with native 7,8-linoleate diol synthase |
Gaeumannomyces graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
- |
Gaeumannomyces graminis var. graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
bifunctional enzyme with linoleic acid (8R)-dioxygenase and hydroperoxide isomerase activities. The enzyme abstracts the 8-pro-S hydrogen from linoleic acid, which is followed by antarafacial insertion of molecular oxygen at C-8 to generate 8R-hydroperoxylinoleate. The latter is then isomerized to (7S,8S,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate by elimination of the 7-pro-S hydrogen and intramolecular suprafacial insertion of an oxygen atom from the hydroperoxide group |
Gaeumannomyces graminis var. graminis |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
- |
Gaeumannomyces graminis var. avenae |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |
5.4.4.6 | (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate |
expression in Pichia pastoris changes the position and stereospecificity of a hydroperoxide isomerase (the recombinant enzyme forms 5,8(R)-dihydroxylinoleic acid (60% 5S) and 8(R),13-dihydroxyoctadeca-(9E,11E)-dienoic acid) possibly due to N- or O-linked mannosides in the vicinity of the heme group, whereas the (8R)-dioxygenase activity is identical with native 7,8-linoleate diol synthase |
Gaeumannomyces graminis var. avenae |
(7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate |
- |
? |