EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  4.4.1.36 | (2R)-3-(2-[[(2R)-2-amino-2-carboxyethyl]sulfanyl]-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + reduced acceptor |
thioether variant of S-(hercyn-2-yl)-L-cysteine S-oxide |
Mycolicibacterium smegmatis |
(2R)-3-(2-sulfido-2,3-dihydro-1H-imidazol-4-yl)-2-(trimethylazaniumyl)propanoate + pyruvate + ammonia + acceptor |
- |
? |
| 4.4.1.36 | 2-(hydroxysulfanyl)hercynine + reduced acceptor |
- |
Neurospora crassa |
ergothioneine + acceptor + H2O |
- |
? |
| 4.4.1.36 | 2-(hydroxysulfanyl)hercynine + reduced acceptor |
- |
Mycolicibacterium smegmatis |
ergothioneine + acceptor + H2O |
- |
? |
| 4.4.1.36 | 2-(hydroxysulfanyl)hercynine + reduced acceptor |
- |
Neurospora crassa DSM 1257 |
ergothioneine + acceptor + H2O |
- |
? |
| 4.4.1.36 | 2-(hydroxysulfanyl)hercynine + reduced acceptor |
- |
Neurospora crassa ATCC 24698 |
ergothioneine + acceptor + H2O |
- |
? |
| 4.4.1.36 | more |
S-(hercyn-2-yl)-L-cysteine S-oxide is the native EgtE substrate and a sulfenic acid intermediate is involved in the ergothioneine C-S lyase reaction |
Mycolicibacterium smegmatis |
? |
- |
? |
| 4.4.1.36 | more |
the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead |
Schizosaccharomyces pombe |
? |
- |
? |
| 4.4.1.36 | more |
enzyme activity determination by a 1H NMR assay of chemical shift of the imidazole hydrogen atoms and a colorimetric assay by coupling the C-S lyase reaction with lactate dehydrogenas, product determination by high-resolution mass spectrometry and NMR |
Neurospora crassa |
? |
- |
? |
| 4.4.1.36 | more |
the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead |
Schizosaccharomyces pombe 972 |
? |
- |
? |
| 4.4.1.36 | more |
enzyme activity determination by a 1H NMR assay of chemical shift of the imidazole hydrogen atoms and a colorimetric assay by coupling the C-S lyase reaction with lactate dehydrogenas, product determination by high-resolution mass spectrometry and NMR |
Neurospora crassa DSM 1257 |
? |
- |
? |
