EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
- |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
i.e. HycE |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
HycI involved in the C-terminal processing of HycE, the large subunit of the hydrogenase 3 from Escherichia coli |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
HycI is an endopeptidase that is responsible for the C-terminal cleavage of the large subunit of hydrogenase 3 in Escherichia coli (UniProt: A1AER2) |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
the final step of maturation of [NiFe]-hydrogenases involves the activity of an endopeptidase which removes an oligopeptide from the C-terminus of the large subunit. The proteolytic maturation is followed by a conformational change, closing of the metal centre, its assembly with the small hydrogenase subunit and finally in the appearance of enzyme activity. HycI is specific for hydrogenase 3 maturation |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
cleavage occurs at the C-terminal side of the Arg537 (removal of a 32-amino acid peptide from the C-terminus). Nickel incorporation into the HYCE precursor is a prerequisite for processing |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
HycI involved in the C-terminal processing of HycE (UniProt: A1AER2), the large subunit of the hydrogenase 3 from Escherichia coli. Mutational alteration of the amino acid residues neighbouring the cleavage site shows that proteolysis still occurs when chemically similar amino acids are exchanged. Processing is blocked in a variant in which the methionine at the C-terminal side is replaced by a glutamate residue. Truncation of the precursor from the C-terminal end renders variants amenable to maturation even when two-thirds of the extension are removed but abolishes proteolysis upon further deletion of a cluster of six basic amino acids. A construct in which the C-terminal extension from the large subunit of the hydrogenase 2 is fused to the mature part of the large subunit of hydrogenase 3 is neither processed by HycI nor by HybD, the endopeptidase specific for the large subunit of hydrogenase 2 |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
HycI is specific for hydrogenase 3 maturation |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
i.e. HycE (UniProt: A1AER2). Cleavage of HycE is specific in that the maturation of the large subunits of hydrogenases 1 and 2 is not affected in strains lacking hycl |
Escherichia coli |
? |
- |
? |
| 3.4.23.51 | precursor of the large subunit of hydrogenase 3 + H2O |
i.e. hycE. Once the metallocenter [NiMeL] is formed in the HycE precursor, processing by HycI and assembly with the other Hyc subunits takes place |
Escherichia coli |
? |
- |
? |
