EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.4.21.B12 | (Abz)-SKGR-SLIGK(N-[2,4-dintrophenyl]ethylenediamine)-Asp-OH |
fluorescence quenched peptide spanning the PAR-2 activation site, is rapidly cleaved by KLK4 |
Homo sapiens |
? |
- |
? |
3.4.21.B12 | 32 kDa enamelin + H2O |
five cleavage sites on the C-terminal sides of R180, Y205, H206, M219 and R262. Kallikrein 4 digestion of the 32 kDa enamelin generates nine major cleavage products. 12 h of digestion with KLK4, all of the 32 kDa enamelin has been cleaved. Some cleavage products persist after 48 h of digestion |
Sus scrofa |
? |
- |
? |
3.4.21.B12 | 52 kDa prostatic acid phosphatase + H2O |
recombinant self-activated, chimeric enzyme |
Homo sapiens |
33 kDa prostatic acid phosphatase + 19 kDa fragment |
products contain 256 and 73 amino acid residues, respectively |
? |
3.4.21.B12 | 52 kDa prostatic acid phosphatase + H2O |
primary cleavage site is the Arg73-Ser74 bond |
Homo sapiens |
33 kDa prostatic acid phosphatase + 19 kDa fragment |
products contain 256 and 73 amino acid residues, respectively |
? |
3.4.21.B12 | 52 kDa prostatic acid phosphatase + H2O |
inactivation of substrate |
Homo sapiens |
33 kDa prostatic acid phosphatase + 19 kDa fragment |
products contain 256 and 73 amino acid residues, respectively |
? |
3.4.21.B12 | 52 kDa prostatic acid phosphatase + H2O |
enzyme may be involved in the physiological clearance of prostatic acid phosphatase |
Homo sapiens |
33 kDa prostatic acid phosphatase + 19 kDa fragment |
- |
? |
3.4.21.B12 | acetyl-FVQR-4-nitroanilide + H2O |
- |
Homo sapiens |
acetyl-FVQR + 4-nitroaniline |
- |
? |
3.4.21.B12 | amelogenin + H2O |
20 kDa substrate |
Sus scrofa |
amelogenin fragments |
- |
? |
3.4.21.B12 | amelogenin + H2O |
recombinant substrate from pig expressed in Escherichia coli strain XL-1 blue as His-tagged protein |
Sus scrofa |
amelogenin fragments |
product identification by N-terminal sequencing and mass spectrometry |
? |
3.4.21.B12 | amelogenin + H2O |
20 kDa substrate |
Sus scrofa EMSP1 |
amelogenin fragments |
- |
? |