EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.2.1.B36 | 4-nitrophenyl alpha-D-maltohexaoside + H2O |
at an early stage of the reaction only maltose and 4-nitrophenol alpha-D-maltotetraoside, but not maltotetraose are observed. Even as the reaction proceeded, maltotetraose does not appear at all |
Pyrococcus sp. |
4-nitrophenyl alpha-D-maltotetraoside + maltose |
- |
? |
| 3.2.1.B36 | 6-O-maltosyl-beta-cyclodextrin + H2O |
- |
Pyrococcus sp. |
maltose + beta-cyclodextrin |
- |
? |
| 3.2.1.B36 | 6-O-maltosyl-beta-cyclodextrin + H2O |
the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible |
Thermococcus sp. |
maltose + beta-cyclodextrin |
- |
? |
| 3.2.1.B36 | 6-O-maltotetraosyl-beta-cyclodextrin + H2O |
- |
Thermococcus sp. |
2 maltose + beta-cyclodextrin |
- |
? |
| 3.2.1.B36 | 6-O-maltotetraosyl-beta-cyclodextrin + H2O |
no formation of matotetraose |
Pyrococcus sp. |
2 maltose + beta-cyclodextrin |
- |
? |
| 3.2.1.B36 | amylopectin + H2O |
the enzyme only released maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan |
Thermococcus sp. |
maltose + ? |
- |
? |
| 3.2.1.B36 | glycogen + H2O |
the enzyme only released maltose from polymers such as soluble starch, amylopectin, and glycogen, while maltose is rarely detected from reaction with amylose and pullulan |
Thermococcus sp. |
maltose + ? |
- |
? |
| 3.2.1.B36 | maltopentaose + H2O |
- |
Pyrococcus sp. |
2 maltose + D-glucose |
- |
? |
| 3.2.1.B36 | maltopentaose + H2O |
the enzyme displays dual hydrolysis activity toward alpha-1,4- and alpha-1,6-glycosidic linkages, the catalytic efficiency of 6-O-maltosyl-beta-cyclodextrin is 16fold higher than that of maltotriose. Compared to the kcat/Km value toward maltotriose, the values for longer substrates such as maltotetraose and maltopentaose are negligible |
Thermococcus sp. |
2 maltose + D-glucose |
- |
? |
| 3.2.1.B36 | maltotetraose + H2O |
- |
Pyrococcus sp. |
2 maltose |
- |
? |
