EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  3.2.1.212 | fucoidan + H2O |
fucoidan from Korean Undaria pinnatifida sporophylls. Endo-acting fucoidanase. The strain depolymerizes fucoidan into more than 7 distinct low molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3749 Da |
Sphingomonas paucimobilis |
? |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
the enzyme catalyzes the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase does not hydrolyze carrageenan. Desulfated fucoidan from Fucus evanescens is hydrolysed very weakly in contrast to deacetylated fucoidan, which is hydrolysed more actively compared to the native fucoidan from Fucus evanescens. Analysis of the structure of the enzymatic products shows that the enzyme shows an endo-type action that is specific for (1->4)-bonds in a polysaccharide molecule built up of alternating 3- and 4-linked alpha-L-fucopyranose residues sulfated mainly at position 2 |
Formosa algae |
? |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
fucoidan from Korean Undaria pinnatifida sporophylls. Endo-acting fucoidanase. The strain depolymerizes fucoidan into more than 7 distinct low molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3749 Da |
Sphingomonas paucimobilis PF-1 |
? |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
fucoidan from the brown alga Pelvetia canaliculata. End products include a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of alpha-(1->3)-L-fucopyranose-2-sulfate-alpha-(1->4)-L-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end |
bacterium |
alpha-L-Fucp-2,3-(diOSO3)-(1->3)-alpha-L-Fucp-2-(OSO3)-(1->4)-alpha-L-Fucp-2,3-(diOSO3)-(1->3)-alpha-L-Fucp-2-(OSO3) + alpha-L-Fucp-2,3-(diOSO3)-(1->3)-alpha-L-Fucp-2-(OSO3)-(1->4)-alpha-L-Fucp-2,3-(diOSO3)-(1->3)-alpha-L-Fucp-2-(OSO3)-(1->4)-alpha-L-Fucp-2,3-(diOSO3)-(1->3)-alpha-L-Fucp-2-(OSO3) + higher molecular-weight fucoidan oligosaccharides |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action |
Haliotis sp. |
sulfated alpha-L-fucooligosaccharides |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively |
Formosa algae |
sulfated alpha-L-fucooligosaccharides |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively |
Formosa algae KMM 3553T |
sulfated alpha-L-fucooligosaccharides |
- |
? |
| 3.2.1.212 | fucoidan + H2O |
the fucoidanase catalyses the hydrolysis of 1->3/1->4-alpha-L-fucans from Fucus evanescens and Fucus vesiculosus, but not 1->3-alpha-L-fucan from Saccharina cichorioides. It catalyzes the hydrolysis of 1->4-bonds in a fucoidan molecule as endo-enzyme |
Lambis sp. |
sulphated fucoidan oligosaccharides with different degree of polymerization |
- |
? |
| 3.2.1.212 | more |
no degradation of 4-nitrophenyl-L-fucoside or blood group substance |
Haliotis sp. |
? |
- |
? |
| 3.2.1.212 | more |
unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) |
Formosa algae |
? |
- |
? |
