EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
    2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Methanocaldococcus jannaschii |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Xanthomonas oryzae pv. oryzae |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Bacillus subtilis |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Saccharomyces cerevisiae |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Methanotorris igneus |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Bacillus subtilis |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
product is a dearomatized thiazole tautomer, not thiazole phosphate |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
ThiG alone can catalyze the formation of thiazole phosphate |
Bacillus subtilis |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] |
- |
Xanthomonas oryzae pv. oryzae ZJ173 |
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O |
- |
? |
| 2.8.1.10 | more |
the in vitro thiazole synthase activity of cleared lysates or desalted proteins is dependent upon the addition of purified ThiG/ThiH-His complex, tyrosine, and an as yet unidentified intermediate present in the protein fraction from the cells |
Escherichia coli |
? |
- |
? |
| 2.8.1.10 | more |
the thiazole-forming activity requires four proteins isolated as heterodimers: ThiGH and ThiFS. Reconstitution of the [4Fe-4S] cluster in ThiH is essential for activity, as is the use of ThiS in the thiocarboxylate form. S-adenosylmethionine binds to the [4Fe-4S] cluster, which becomes more susceptible to reduction to the +1 state. In addition to the proteins, 1-deoxy-xylulose-5-phosphate, tyrosine, S-adenosylmethionine, and a reductant are required. 1 mol equivalent of thiazole phosphate is formed per ThiGH. For each mole of thiazole phosphate formed, 1 equivalent of S-adenosylmethionine and 1 equivalent of tyrosine are utilized, and 1 equivalent of 5-deoxyadenosine is produced. Mechanistic hypothesis suggests that S-adensylmethionine is reductively cleaved to yield a highly reactive 5-deoxyadenosyl radical. This radical abstracts the phenolic hydrogen atom from tyrosine, and the resultant substrate radical cleaves to yield dehydroglycine, which is required by ThiG for the thiazole cyclization reaction |
Escherichia coli |
? |
- |
? |
