EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.4.1.37 | beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + UDP-GalNAc |
- |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
2 distinct alpha-3-D-galactosyltransferases: one which is more tightly membrane-bound, resembles the human B-gene-specific transferase in its acceptor specificity, and the second, which is a more soluble enzyme transfers D-galactose to the same positional linkage in unsubstituted beta-D-galactosyl residues |
Oryctolagus cuniculus |
? |
- |
? |
| 2.4.1.37 | more |
substrate specificity, enzyme forms Galalpha1,3Fuc-linkages, specific for L-fucose residues |
Dictyostelium discoideum |
? |
- |
? |
| 2.4.1.37 | more |
fragment-based screening of the donor substrate specificity. Enzyme binds several UDP-activated sugars, including UDP-Glc, UDP-GlcNAc, and UDP-GalNAc. In all cases, UDP is the dominant binding epitope. The binding of donor substrate to GTB is essentially controlled by the base as a molecular anchor. Uracil represents the smallest fragment that is recognized. CDP, AMP, and GDP do not exhibit any significant binding affinity for the enzyme. The ribose and beta-phosphate moieties increase the affinity of the ligands. The pyranose sugar weakens the binding, although this part of the molecule controls the specificity of the enzyme. UDP represents the best binder. The binding affinities of UDP-Gal, UDP-Glc, and UMP are about the same, but lower than that of UDP. beta-D-Galactose and alpha-D-galactose bind weakly to GTB. Whereas beta-D-galactose binds to the acceptor and donor sites, it is suggested that alpha-D-galactose occupies a third hitherto unknown binding pocket |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
development and evaluation of a general screening assay method for glycosyltransferase activities, overview |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
histo-blood group B transferase transfers alpha-D-galatose to the antigen,H, while the B type enzyme transfers N-acetylgalactosamine, overview |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
the enzyme shows weakened activity with B antigen variants resulting from ABO polymophisms, phenotyping and genotyping of ABO antigens, overview |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
enzyme-substrate interaction analysis by electrospray ionization mass spectrometry, binding of substrate and product analogues, overview |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
galactose is used as an acceptor analogue and UDP as a donor analogue in all soaking trials |
Homo sapiens |
? |
- |
? |
| 2.4.1.37 | more |
mouse ABO gene-encoded cis-AB transferase contains the GlyGlyAla tripeptide sequence, which is also found in the majority of GBGT1 genes encoding Forssman glycolipid synthase, EC 2.4.1.88. Mouse cis-AB transferase with the GlyGlyAla tripeptide sequence, but not the human cis-AB transferase with the LeuGlyAla, exhibits Forssman glycolipid synthase activity equivalent of mouse GBGT1 gene-encoded Forssman glycolipid synthase |
Mus musculus |
? |
- |
- |
