EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet |
- |
Thermoplasma acidophilum |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in elongator tRNAMet |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet |
- |
Thermoplasma acidophilum HO-62 |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in elongator tRNAMet |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet |
- |
Thermoplasma acidophilum |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet |
- |
Thermoplasma acidophilum HO-62 |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in initiator tRNAMet |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
- |
Pyrococcus horikoshii |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
- |
Saccharomyces cerevisiae |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group |
Pyrococcus furiosus |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
the efficient dimethylation of guanine26 requires the presence of base-pairs C11*G24 and G10*C25 and a variable loop of five bases within a correct 3D-core of the tRNA molecule. These identity elements probably ensure the correct presentation of methylated m2G26 to the enzyme for the attachment of the second methyl group. The methylated intermediate, and the enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions |
Pyrococcus furiosus |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
the elements necessary for a productive interaction between G26 in nuclear coded yeast tRNAs and the yeast G26 modifying enzyme are located within the core of the tRNA |
Saccharomyces cerevisiae |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |
2.1.1.216 | 2 S-adenosyl-L-methionine + guanine26 in tRNA |
wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA |
Caenorhabditis elegans |
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA |
- |
? |