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EC Number
Substrates
Commentary Substrates
Organism
Products
Commentary (Products)
Reversibility
1.8.1.15
2 mycothiol + NADP+
-
Corynebacterium glutamicum
mycothione + NADPH + H+
-
r
1.8.1.15
2,6-dimethylbenzoquinone + NADPH
-
Mycobacterium tuberculosis
?
-
?
1.8.1.15
2,6-dimethylbenzoquinone + NADPH
-
Mycobacterium tuberculosis H37Rv
?
-
?
1.8.1.15
2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside disulfide + NADPH
-
Mycobacterium tuberculosis
?
-
?
1.8.1.15
2-methyl-1,4-naphthoquinone + alpha-NADPH
-
Mycobacterium tuberculosis
?
-
?
1.8.1.15
5,8-dihydroxy-1,4-naphthoquinone + NADPH
-
Mycobacterium tuberculosis
?
-
?
1.8.1.15
5-(benzyl 2-(-N-acetyl-L-cysteinyl) amino-2-deoxy-alpha-D-glucopyranoside)-dithio-2-nitrobenzoate + NADPH
a 5,5'-dithiobis-2-nitrobenzoic acid (DTNB)-coupled assay is developed. The mixed disulfide substrate liberates one molecule of TNB on NADPH-dependent reduction by Mycobacterium tubercolosis reductase. The liberated mycothiol analogue then reacts with DTNB to regenerate the mixed disulfide substrate and another molecule of TNB. Reaction progress can be measured by the increase in absorbance (412 nm) from the two molecules of TNB produced per turn of this catalytic cycle
Mycobacterium tuberculosis
?
-
?
1.8.1.15
5-hydroxy-1,4-naphthoquinone + NADPH
-
Mycobacterium tuberculosis
?
-
?
1.8.1.15
5-hydroxy-1,4-naphthoquinone + NADPH
-
Mycobacterium tuberculosis H37Rv
?
-
?
1.8.1.15
7-methyljuglone + NADPH
potent subversive substrate
Mycobacterium tuberculosis
?
-
?
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