EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.99.15 | 2-naphthoic acid + AH2 + O2 |
only the wild-type enzyme hydroxylates 2-naphthoic acid at the C-7 and C-8 positions, whereas all of the active F185 mutants exhibit a preference for C-5 hydroxylation |
Rhodopseudomonas palustris |
5-hydroxy-2-naphthoic acid + A + H2O |
- |
? |
| 1.14.99.15 | 2-naphthoic acid + AH2 + O2 |
only the wild-type enzyme hydroxylates 2-naphthoic acid at the C-7 and C-8 positions, whereas all of the active F185 mutants exhibit a preference for C-5 hydroxylation |
Rhodopseudomonas palustris |
7-hydroxy-2-naphthoic acid + A + H2O |
- |
? |
| 1.14.99.15 | 2-naphthoic acid + AH2 + O2 |
only the wild-type enzyme hydroxylates 2-naphthoic acid at the C-7 and C-8 positions, whereas all of the active F185 mutants exhibit a preference for C-5 hydroxylation |
Rhodopseudomonas palustris |
8-hydroxy-2-naphthoic acid + A + H2O |
- |
? |
| 1.14.99.15 | 3,4-dimethoxybenzoate + NADH + O2 |
- |
Pseudomonas putida |
4-hydroxy-3-methoxybenzoate + NAD+ + H2O + formaldehyde |
- |
? |
| 1.14.99.15 | 3,4-methylenedioxybenzoate + NADH + O2 |
piperonylate |
Pseudomonas putida |
3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde |
protocatechuate |
? |
| 1.14.99.15 | 3-chlorobenzoic acid + NADH + O2 |
- |
Rhodopseudomonas palustris |
? + NAD+ + H2O |
- |
? |
| 1.14.99.15 | 3-hydroxybenzoate + NADH + O2 |
partial uncoupler |
Pseudomonas putida |
3,4-dihydroxybenzoate + NAD+ + H2O |
in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too |
? |
| 1.14.99.15 | 3-methoxybenzoate + NADH + O2 |
partial uncoupler |
Pseudomonas putida |
3-hydroxybenzoate + NAD+ + H2O + formaldehyde |
- |
? |
| 1.14.99.15 | 3-methoxybenzoate + NADH + O2 |
partial uncoupler |
Pseudomonas putida |
3-hydroxybenzoate + NAD+ + H2O + formaldehyde |
in the uncoupled part of the reaction 3-methoxybenzoate is not hydroxylated, and H2O2 is a product, too |
? |
| 1.14.99.15 | 3-nitro-4-methoxybenzoate + NADH + O2 |
- |
Pseudomonas putida |
3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde |
- |
? |
