EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.15.35 | (9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
- |
Saccharopolyspora erythraea |
(9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | (9R)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
hydroxylation at a rate approximately 2fold lower than the natural substrate 6-deoxyerythronolide B |
Saccharopolyspora erythraea |
(9R)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | (9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
- |
Saccharopolyspora erythraea |
(9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | (9S)-9-deoxo-9-hydroxy-6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
hydroxylation at a rate approximately equal to the natural substrate 6-deoxyerythronolide B |
Saccharopolyspora erythraea |
(9S)-9-deoxo-9-hydroxyerythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | (9S)-9-deoxo-9-hydroxy-8,8a-deoxyoleandolide + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
substrate is identical to 6-deoxyerythronolide B except for the presence of a C13 methyl group. Hydroxylation at a rate approximately 4fold lower than the natural substrate 6-deoxyerythronolide B |
Saccharopolyspora erythraea |
6-hydroxy-8,8a-deoxyoleandolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
- |
Saccharopolyspora erythraea |
erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
the enzyme is involved in the biosynthesis of the antibiotic erythromycin |
Saccharopolyspora erythraea |
erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
direct involvement of the substrate in O2 activation |
Saccharopolyspora erythraea |
erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
the enzyme is involved in the biosynthesis of the antibiotic erythromycin |
Saccharopolyspora erythraea NRRL 2338 |
erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.35 | 6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
direct involvement of the substrate in O2 activation |
Saccharopolyspora erythraea NRRL 2338 |
erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |