EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.36 | 1-nitro-2-(4-hydroxyphenyl)ethane + O2 + [reduced NADPH-hemoprotein reductase] |
involvement of 1-nitro-2-(4-hydroxyphenyl)ethane or more likely its aci-nitro tautomer as an intermediate between N-hydroxytyrosine and 4-hydroxyphenylacetaldehyde oxime |
Sorghum bicolor |
N,N-dihydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
- |
Sorghum bicolor |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
- |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
- |
Triglochin maritima |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
- |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
- |
Sorghum bicolor |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
overall reaction |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
- |
Sorghum bicolor |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
overall reaction. NADPH is a much better cofactor for NADPH-hemoprotein reductase than NADH although NADH does support the entire catalytic cycle |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
intermediate products: N-hydroxytyrosine, N,N-dihydroxytyrosine, (E)-p-hydroxyphenylacetaldoxime |
Sorghum bicolor |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
- |
? |
1.14.14.36 | L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] |
overall reaction, P450Tyr is a multifunctional tyrosine N-hydroxylase catalyzing the double N-hydroxylation of L-tyrosine to N,N-dihydroxy-L-tyrosine which dehydrates and decarboxylates to 4-hydroxyphenylacetaldoxime |
Sorghum bicolor |
(E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O |
- |
? |
1.14.14.36 | L-tyrosine + O2 + NADH + H+ |
- |
Sorghum bicolor |
N-hydroxy-L-tyrosine + NAD+ + H2O |
- |
r |
1.14.14.36 | L-tyrosine + O2 + NADPH + H+ |
- |
Sorghum bicolor |
N-hydroxy-L-tyrosine + NADP+ + H2O |
- |
r |
1.14.14.36 | L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] |
- |
Sorghum bicolor |
N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |