EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.14.176 | more |
the recombinant enzyme produces 5(12)-oxa-3(11)-cyclotaxane, a tritiated oxidized taxane, instead of taxa-4(20),11-dien-5alpha-ol, structural analysis of the compound, overview |
Taxus cuspidata |
? |
- |
? |
1.14.14.176 | more |
cDNAs encoding taxa-4(5),11(12)-diene synthase and CYP725A4 are introduced in Nicotiana sylvestris for specific expression in trichome cells. The co-expression does not lead to the production of the expected 5-hydroxytaxa-4(20),11(12)-diene. Instead, taxa-4(5),11(12)-diene is quantitatively converted to a novel taxane |
Taxus cuspidata |
? |
- |
? |
1.14.14.176 | taxa-4(20),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2 |
preferred substrate |
Taxus cuspidata |
taxa-4(20),11(12)-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.176 | taxa-4(20)-11(12)-diene + AH2 + O2 |
- |
Taxus sp. |
? |
- |
? |
1.14.14.176 | taxa-4(5),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Taxus cuspidata |
taxa-4(20),11(12)-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.176 | taxa-4(5),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2 |
hydroxylation of the C-5alpha is the first of the oxidations resulting in taxol |
Taxus cuspidata |
5(12)-oxa-3(11)-cyclotaxane + [oxidized NADPH-hemoprotein reductase] + H2O |
OCT is a cyclic ether |
? |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Taxus cuspidata |
taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
Taxus brevifolia |
taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
reaction is completely dependent on NADPH |
Taxus cuspidata |
taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |
1.14.14.176 | taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2 |
reaction is completely dependent on NADPH |
Taxus brevifolia |
taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O |
- |
? |