EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.12.12 | (1S)-indanol + NADH + O2 |
recombinant enzyme |
Pseudomonas sp. |
(1S)-indanol + trans-1,3-indandiol |
85.5% indanol, 11.5% 1,3-indandiol, minor products: (1S)-indenol and 1-indanone |
? |
| 1.14.12.12 | (2S)-flavanone + NADH + H+ + O2 |
no substrate for wild-type, substrate for mutants F224C, F224K, F224Q, F224Y |
Pseudomonas sp. |
8-hydroxy-(2S)-flavanone + NAD+ + ? |
product has an oxygen atom directly attached to the aromatic ring. Upon completion of the dihydroxylation, the oxygen atom on the C-ring is believed to expel the 7-OH group through donation of its lone pair electrons and the charged intermediate. Once formed this readily re-aromatizes to provide 8-hydroxyflavanones or 8-hydroxyisoflavanones |
? |
| 1.14.12.12 | (2S)-flavanone + NADH + H+ + O2 |
no substrate for wild-type, substrate for mutants F224C, F224K, F224Q, F224Y |
Pseudomonas sp. NCIB 9816-4 |
8-hydroxy-(2S)-flavanone + NAD+ + ? |
product has an oxygen atom directly attached to the aromatic ring. Upon completion of the dihydroxylation, the oxygen atom on the C-ring is believed to expel the 7-OH group through donation of its lone pair electrons and the charged intermediate. Once formed this readily re-aromatizes to provide 8-hydroxyflavanones or 8-hydroxyisoflavanones |
? |
| 1.14.12.12 | (3S)-isoflavanone + NADH + H+ + O2 |
no substrate for wild-type, substrate for mutants F224K, F224Y |
Pseudomonas sp. |
8-hydroxy-(3S)-isoflavanone + NAD+ + ? |
product has an oxygen atom directly attached to the aromatic ring. Upon completion of the dihydroxylation, the oxygen atom on the C-ring is believed to expel the 7-OH group through donation of its lone pair electrons and the charged intermediate. Once formed this readily re-aromatizes to provide 8-hydroxyflavanones or 8-hydroxyisoflavanones |
? |
| 1.14.12.12 | (3S)-isoflavanone + NADH + H+ + O2 |
no substrate for wild-type, substrate for mutants F224K, F224Y |
Pseudomonas sp. NCIB 9816-4 |
8-hydroxy-(3S)-isoflavanone + NAD+ + ? |
product has an oxygen atom directly attached to the aromatic ring. Upon completion of the dihydroxylation, the oxygen atom on the C-ring is believed to expel the 7-OH group through donation of its lone pair electrons and the charged intermediate. Once formed this readily re-aromatizes to provide 8-hydroxyflavanones or 8-hydroxyisoflavanones |
? |
| 1.14.12.12 | (R)-1-indanol + NADH + H+ + O2 |
activity in E. coli cells expressing recombinant naphthalene dioxygenase |
Pseudomonas sp. |
cis-1,3-indandiol + (R)-3-hydroxy-1-indanone + cis-1,2,3-indantriol + NAD+ |
71% cis-1,3-indandiol, 18.2% (R)-3-hydroxy-1-indanone and 10.8% cis-1,2,3-indantriol |
? |
| 1.14.12.12 | (S)-1-indanol + NADH + H+ + O2 |
activity in E. coli cells expressing recombinant naphthalene dioxygenase |
Pseudomonas sp. |
trans-(1S,3S)-indan-1,3-diol + (R)-3-hydroxy-1-indanone + NAD+ |
95.5% trans-(1S,3S)-indan-1,3-diol |
? |
| 1.14.12.12 | (S)-1-indenol + NADH + O2 |
recombinant enzyme system |
Pseudomonas sp. |
syn-2,3-dihydroxy-1-indanol + anti-2,3-dihydroxy-1-indanol |
- |
? |
| 1.14.12.12 | 1,2,4-trimethylnaphthalene + NADH + H+ + O2 |
- |
Ralstonia sp. |
? + NAD+ |
generation of mono- and dioxygenated products |
? |
| 1.14.12.12 | 1,2,4-trimethylnaphthalene + NADH + H+ + O2 |
- |
Ralstonia sp. U2 |
? + NAD+ |
generation of mono- and dioxygenated products |
? |
