EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.1.1.86 | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ |
- |
Escherichia coli |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ |
- |
r |
1.1.1.86 | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ |
- |
Oryza sativa |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ |
- |
r |
1.1.1.86 | (2S)-2-aceto-2-hydroxybutyrate + NADPH + H+ |
- |
Corynebacterium glutamicum |
(R)-2,3-dihydroxy-3-methylvalerate + NADP+ |
- |
? |
1.1.1.86 | (2S)-2-aceto-2-hydroxybutyrate + NADPH + H+ |
- |
Corynebacterium glutamicum ATCC 13032 |
(R)-2,3-dihydroxy-3-methylvalerate + NADP+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADH + H+ |
the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH |
Corynebacterium glutamicum |
(R)-2,3-dihydroxyisovalerate + NAD+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADH + H+ |
the enzyme prefers NADPH as a cofactor rather than NADH. kcat/Km for NADPH is 322fold higher than kcat/KM for NADH |
Corynebacterium glutamicum ATCC 13032 |
(R)-2,3-dihydroxyisovalerate + NAD+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADPH |
- |
Staphylococcus aureus |
(2R)-2,3-dihydroxyisovalerate + NADP+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADPH |
- |
Staphylococcus aureus NCTC 8325 |
(2R)-2,3-dihydroxyisovalerate + NADP+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADPH + H+ |
- |
Mycobacterium tuberculosis |
(2R)-2,3-dihydroxyisovalerate + NADP+ |
- |
? |
1.1.1.86 | (2S)-2-acetolactate + NADPH + H+ |
the reaction includes the initial deprotonation of the substrate C2 hydroxy group, bridged by the two Mg2+ ions, alkyl migration from the C2-alkoxide carbon atom to the C3-carbon |
Staphylococcus aureus |
(2R)-2,3-dihydroxyisovalerate + NADP+ |
- |
? |