EC Number   |
Substrates   |
Organism   |
Products   |
Reversibility   |
---|
  4.2.1.34 | mesaconate + H2O |
- |
Escherichia coli |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
- |
Paraburkholderia xenovorans |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase |
Paraburkholderia xenovorans |
(S)-citramalate |
- |
? |
  4.2.1.34 | mesaconate + H2O |
heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate |
Escherichia coli |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate |
Escherichia coli |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate |
Escherichia coli |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
- |
Paraburkholderia xenovorans DSMZ 17367 / LB400 |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase |
Paraburkholderia xenovorans DSMZ 17367 / LB400 |
(S)-citramalate |
- |
? |
  4.2.1.34 | mesaconate + H2O |
- |
Escherichia coli ATCC 700728 / O157:H7 |
(S)-citramalate |
- |
r |
  4.2.1.34 | mesaconate + H2O |
heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate |
Escherichia coli ATCC 700728 / O157:H7 |
(S)-citramalate |
- |
r |