3 sucrose + sucrose
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3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
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3 sucrose + sucrose
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(2â1) linkages]
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
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?
3 sucrose + sucrose
enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(2â1) linkages]
3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
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more
the enzyme is able to catalyse a disproportionation type of reaction with 1-kestose, 1,1-nystose and 1,1,1-kestopentaose
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more
the production of fructooligosaccharide by this enzyme is possible only in the presence of sucrose which is an exclusive donor of fructosyl residue in the transferase reaction. The acceptor can be another molecule of sucrose or frucotoligosaccharide
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more
wild-type inulosucrase synthesizes mostly fructooligosaccharides up to a degree of polymerization of 15 and relatively low amounts of inulin polymer
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more
very low activity in acceptor reactions with non-sugar acceptors, specificity analysis, overview
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more
synthesis of 1-kestose and nystose
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more
enzymatic synthesis of maltosylfructosides derived from sucrose-maltose mixtures by the enzyme from strain DSM 20604, structure analysis, and relation to fructooligosaccharides contents, overview
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more
sucrose or raffinose act as substrates, both donor and acceptor of fructose moieties. Sucrose is the preferred substrate. The enzyme synthezises fructooligosaccharides from degree of polymerization of 3 (1-kestose) to 9, as well as minor amounts of neo-kestose and inulobiose, product analysis by NMR spectroscopy, overview. Synthezised oligomers are identified as alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside, and alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranosyl-(1->2)-beta-D-fructofuranoside
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