EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.241 | more |
the only substance that serves as a substrate for the recombinant Bx7 is TRIBOA-Glc, neither the aglucone TRIBOA nor well-known substrates of plant O-methyltransferases are efficiently converted by the enzyme. In case of quercetin, a minor activity is detected. BX7 has no catalytic activity toward phenylpropanoids and flavonoids, e.g. apigenin is no substrate |
Zea mays |
? |
- |
? |
2.1.1.241 | S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside |
- |
Zea mays |
S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside |
- |
? |
2.1.1.241 | S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside |
conversion of (2R)-2,4,7-trihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside, TRIBOA-Glc, to DIMBOA-Glc |
Zea mays |
S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside |
i.e. DIMBOA-Glc |
? |
2.1.1.241 | S-adenosyl-L-methionine + 2-(2,4,7-trihydroxy-8-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose |
i.e. TRIMBOA-Glc |
Zea mays |
S-adenosyl-L-homocysteine + 2-(2,4-dihydroxy-7,8-dimethoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose |
- |
? |
2.1.1.241 | S-adenosyl-L-methionine + 2-(2,4,7-trihydroxy-8-methoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose |
i.e. TRIMBOA-Glc, enzyme BX7 methylates the C-7 hydroxyl group of TRIBOA-Glc. Recombinant BX7 also accepts TRIMBOA-Glc as substrate and methylates the hydroxyl group at C-7 |
Zea mays |
S-adenosyl-L-homocysteine + 2-(2,4-dihydroxy-7,8-dimethoxy-1,4-benzoxazin-3-one)-beta-D-glucopyranose |
- |
? |