1-methylisatin + NADPH + H+
86% of the activity compared to isatin
?
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
ir
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
69% of the activity compared to isatin. The stereospecificity for NADPH is not known. Activity with NADH is 2% compared to the activity with NADPH
(R)-pantolactone + NADP+
-
ir
2-dehydropantolactone + NADPH + H+
recombinant CorCPR exhibited a high catalytic activity for the synthesis of (R)-pantolactone ((R)-PL) from 2-dehydropantolactone (KPL). The activity assay is performed using purified CorCPR and crude recombinant GDH in a single batch reaction. GDH is used to complete the enzyme-coupled NADPH regeneration system. CPR-01 form Candida orthopsilosis Co 90-125 exhibits excellent enantioselectivity (enantiomeric excess of over 99%). CorCPR exhibits the highest activity and stereoselectivity toward KPL
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
(R)-pantolactone + NADP+
-
?
