EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
Cfr is an plasmid-acquired methyltransferase that protects cells from the action of antibiotics |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
S-adenosylmethionine is both the methyl donor and the source of a 5'-deoxyadenosyl radical, which activates the substrate for methylation. The enzyme can utilize protein-free 23S rRNA as a substrate, but not the fully assembled large ribosomal subunit, suggesting that the methylations take place during the assembly of the ribosome. The key recognition elements in the 23S rRNA are helices 90-92 and the adjacent single stranded RNA that encompasses A2503 |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
identification of the reaction products as 8-methyladenine2503, and 2,8-dimethyladenine2503 |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
the newly introduced methyl group is assembled from an S-adenosyl-L-methionine (SAM)-derived methylene fragment and a hydrogen atom that had migrated from the substrate amidine carbon. Rather than activating the adenosine nucleotide of the substrate by hydrogen atom abstraction from an amidine carbon, the 5'-deoxyadenosyl radical abstracts hydrogen from the second equivalent of SAM to form the SAM-derived radical cation. This species, or its corresponding sulfur ylide, subsequently adds into the substrate, initiating hydride shift and S-adenosylhomocysteine elimination to complete the formation of the methyl group |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
the enzyme methylates the 8 position of 23S rRNA residue A2503 to confer resistance to multiple antibiotic classes acting upon the large subunit of the bacterial ribosome |
Azotobacter vinelandii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
in the presence of sodium dithionite reconstituted Cfr is both reducible and able to cleave S-adenosyl-L-methionine to 5'-deoxyadeonsine, DOA |
Azotobacter vinelandii |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Bacillus amyloliquefaciens FZB42 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Bacillus amyloliquefaciens FZB42 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
verification of RNA methylation at A2503 in 23S rRNA by primer extension method |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Brevibacillus brevis NBRC 100599 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Brevibacillus brevis NBRC 100599 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
verification of RNA methylation at A2503 in 23S rRNA by primer extension method |
? |
| 2.1.1.224 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Staphylococcus aureus RN4220 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA |
- |
? |
