cis-3-(acetyloxy)-4-phenyl-2-azetidinone + H2O
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(3R,4S)-cis-3-(acetyloxy)-4-phenyl-2-azetidinone + (3S,4R)-cis-3-hydroxy-4-phenyl-2-azetidinone + acetate
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maximal activity with fatty acid chain length of 14 carbon atoms, similarly active with the diacylglycerols and triacylglycerols and more active with oleic acid than with stearic acid
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1,3-positional specific lipase
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the enzyme prefers triglycerides with C16-fatty acyl chains and C18-fatty acyl chains, it cleaves only the primary groups of triglycerides, marked preference for substrates containing endogenously occuring fatty acids
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highly active against all triacylglycerols and very low activity against partial acylglycerols or monoesters
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the enzyme hydrolyzes triacylglycerols to fatty acids and glycerol, the fatty acids from the sn-1 or 3-positions are hydrolyzed first to yield 1,2(2,3)-diacylglycerols
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specific for the primary position of the glycerides. Triglycerides with C18 are hydrolyzed in this order: C18:3, C18:2, C18:1, C18:0
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methyl esters are cleaved by lipase 5 times faster than ethyl esters. The relative hydrolysis rates are rather low for esters of C3 and C4 alcohols, they increase abruptly for esters of n-hexyl alcohol, which are cleaved faster than methyl esters
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no activity with 4-nitrophenyl acetate
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