EC Number   |
Storage Stability |
|---|
    3.6.1.11 | -20°C, 50 mM Tris-HCl buffer, pH 7.6, 10 mM MgCl2, 0.5 mM EDTA, 150 mM NaCl, 20% loss of activity after 2 months |
| 3.6.1.11 | -20°C, in 50 mM Tris-HCl buffer, pH 7.5, 5 mM MgCl2, 0.5 mM EDTA, 50 mM NaCl, 0.1% w/v bovine serum albumin, 40% v/v glycerol, exopolyphosphatase I and II, less than 30% loss of activity after 2 months |
| 3.6.1.11 | -20°C, stable for months |
| 3.6.1.11 | -4°C, 0.1% Triton X-100, 1M KCl, 4 days, no activity loss |
| 3.6.1.11 | -4°C, enzyme desorbed from heparin-agarose with 1 M KCl and 0.1% Triton X-100, 4 days, no loss of activity |
| 3.6.1.11 | -80°C, 300 mM NaCl, 250 mM sucrose, 2 mM dithiothreitiol, several months, no activity loss |
| 3.6.1.11 | 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months |
| 3.6.1.11 | 4°C, enzyme obtained by desorption with polyphosphate, 2 h, 30% loss of activity |
| 3.6.1.11 | 4°C, in absence of detergents, complete loss of activity after 24 h, can be stabilized by 0.1% Triton X-100 and protease inhibitors |
| 3.6.1.11 | 92°C, in the presence of 0.1% Triton X-100, 5 days, 8% loss of activity of the partially purified exopolyphosphatase preparation after DEAE-Toyopearl chromatography |
