EC Number |
Storage Stability |
---|
1.5.1.5 | -17°C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol, |
1.5.1.5 | -20°C, 0.1% bovine plasma albumin |
1.5.1.5 | -20°C, 1 month, residual activity of 50% |
1.5.1.5 | -20°C, purified MTHFD2L in 25 mM Tris-Cl, 10 mM 2-mercaptoethanol, 100mM KCl, and 50% v/v glycerol at pH 7.5. The purified enzyme retains about 75% of initial 5,10-CH2THF dehydrogenase activity after 1 week of storage |
1.5.1.5 | -20°C, stable for at least 2 months at diluted protein concentration |
1.5.1.5 | -20°C, stable fot at least 2 months |
1.5.1.5 | 0-4°C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity |
1.5.1.5 | 10% glycerol required to prevent loss of activity during storage |
1.5.1.5 | 2-4°C, 10% loss of activity in 4 days |
1.5.1.5 | 4°C, in the presence of 25% glycerol, 1 month, residual activity of 85% |