EC Number   |
Storage Stability |
|---|
   1.2.4.4 | -20°C, after concentration and dialysis into 50 mM Tris-HCl buffer, 0.1 mM EDTA, 0.1 mM EGTA, 1 mM benzamidine, 1 mM PMSF, 1 mM DTT, pH 7.3, multienzyme complex is stable for at least 2 months |
| 1.2.4.4 | -70°C, 10 mM Tris-HC1, pH 8.0, containing 0.01 mM EDTA, 0.2 M NaCI, 0.005% (v/v) 2-mercaptoethanol, and 50% (v/v) glycerol, 2 years, remains active |
| 1.2.4.4 | -70°C, 50 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.2 mM thiamine diphosphate, 0.2 mM phenylmethylsulfonyl fluoride, stable for at least 3 weeks |
| 1.2.4.4 | -70°C, enzyme complex is stable for more than 8 months |
| 1.2.4.4 | -70°C, multienzyme complex is stable for at least 2 years |
| 1.2.4.4 | 0-5°C, rapid loss of 20-30% of the activity, then stable for 3-4 months |
| 1.2.4.4 | 0°C, glycerol buffer, 20% loss of activity after 24 h |
